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Starting with Enzyme Technology Introduction, Enzymes are proteins whose biological function is to catalyze the reactions that occur in cells, although they are also capable of catalyzing reactions with non-natural substrates. In addition to this characteristic, enzymes have a high selectivity to recognize substrates, they work under mild conditions, and, being biological macromolecules, they do not alter the environment. Therefore, it is not surprising that these biocatalysts are presented as a very interesting alternative to chemical catalysts. Specifically, microorganism enzymes have proven usefulness in obtaining detergents, food additives, chemicals, and pharmaceuticals on a laboratory scale.
Enzyme technology aims to overcome all those drawbacks that seem to delay the application of enzymes in these processes on an industrial scale. For example, genetic engineering has allowed the cloning of the genes that code for the synthesis of enzymes and their expression in large quantities, which has forced a reduction in their price. Immobilization techniques have succeeded in improving the stability of enzymes, and have facilitated their recovery and recycling.
Finally, the retention of the catalytic activity of the enzymes in systems with organic solvents has allowed the transformation of many substrates insoluble in water and has opened new routes in obtaining products with high added value.
Enzymes are fundamental pieces in cell survival since they carry a multitude of vital functions. In fact, the malfunction of some enzymes can lead to very serious pathologies such as phenylketonuria or Gaucher disease, as well as being related to other more common disorders such as diabetes or hypertension. However, enzymes can be isolated and used outside the cellular context, as tools for other technological applications.
The isolation of enzymes carries with it the problem of stability since isolated enzymes are usually too labile to be used in technological applications. For this reason, they are formulated with stabilizing agents or incorporated into solid materials (nanoparticles, plastics, minerals??). To increase their useful life outside cells and thus be able to use them in industrial processes. Thanks to these formulations, artificial metabolisms can be reconstructed that bring together a set of enzymes from different organisms. Thus, it is possible to carry out biosynthetic routes that are not found in nature and that give rise to products with high industrial interest.
The recovery from an infection, the satisfaction of our thirst when drinking a soda, as well as the neatness of our garments are largely due to enzyme technology, which has put enzymes at the service of our needs.
Enzymes are the oldest known catalysts, since thousands of years ago, long before their discovery at the end of the 19th century by the German Eduard Buchner, our ancestors already used them, without knowing it, when fermenting wine. Today, all penicillin-derived antibiotics (for example amoxicillin) are made from the production of enzymes based on an enzyme of fungal origin immobilized in a porous solid material. A similar technology, but with another enzyme: glucose isomerize, is used to produce most of the fructose syrups that sweeten sugary drinks. And surely most of us, when we put the washing machine, use the enzyme cocktail in the form of small particles present in almost all detergents.
Besides that, if you are assigned questions about DNA technology see the attached below samples for reference. These samples are available for free once you get registered on our website. Experts of our assignment help explain that Recombinant DNA technology is a technology that uses enzymes to cut and join sequences of DNA of interest. The recombinant DNA sequences can be placed in vehicles called vectors that transport the DNA to the right place in the host cell where it can be copied or expressed.
We have hired highly talented and experienced teaching assistants who can provide any possible assistance in completing enzyme assignments to facilitate understanding of even the most complex concepts related to enzymes.
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