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Do you feel all lost in those extensive biological cascade pathways? Feeling like your DNA has unwound in the attempt to learn how the processes of DNA replication, transcription, and translation work? Or is there a still deeper level of problem you are dealing with, like why the replisomes assemble in the direction they do? What are the forces involved? It’s alright, it’s alright! We get that it’s indeed very complex. After all, it is said that all human DNA could go up to the sun and back over 500 times if put together, end to end. So, naturally, it has to be very intricately designed, which makes it a bit complicated to understand.
This beauty and wonder of creation, however, takes a toll on the students who try to study all the detailed mechanisms of action and synthesis of various important biomolecules of the human body. But what is the worry when you have an entire team of Molecular Biology academic writers at your disposal to help you learn whatever it is you are having a hard time with? With us, availing molecular biology assignment help could not be easier! Let us tell you more about it! Read ahead.
Molecular Biology is an important branch of biology that deals with other biological fields as an interdisciplinary subject. These other fields are mainly concerned with genetics and biochemistry. As the name suggests, the subject tries to explore the workings inside the cells at a molecular level. For this exploration and research, various tools and techniques have developed over the history of molecular biology. Our team of experts who provide molecular biology essay help explain a few of these techniques which you must know before starting to write that molecular biology assessment answer.
PCR is by far the most important and amongst the first developed techniques that found its permanent niche under the umbrella of molecular biology. PCR is a chemical reaction that is exploited to make a large number of copies of a DNA molecule of interest. There are multiple types of PCRs designed with slight modifications. Some of them are:
This is a widely used technique for the genotyping of multiple genes on a genome. It is used to study the expression profile of multiple genes on the DNA at once. In this, sample DNA is loaded in picomolar quantity to a solid surface and each of these is called “spots.” Each spot contains a specific DNA sequence which is known as probes. The microarray analysis essentially produces a profile of the expression levels of various genes which indicate the levels and quantities of proteins synthesized, respective to the gene, which further cues at the cellular and molecular function of the cell. Broadly, DNA microarrays are of four types, wiz. cDNA microarrays, BAC microarrays, oligo DNA microarrays, and SNP microarrays.
Molecular cloning is amongst the pivotal techniques, which most molecular biology revolves around. It deploys the use of the polymerase chain reaction and is used to code DNA for a specific protein of interest and insert it into a plasmid vector that is viable in the target cell or the organism. The resulting molecularly altered plasmid is often integrated into the genome of the organism wherein the target genes are transcribed and the desired gene product is extracted. Molecular Cloning has wide applications ranging from its use in the production of antibiotics, hormones, etc. to bacterial cloning that facilitates the nitrogen fixation capacity in plants.
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